Related Experiment Videos
[The regulating mechanism of anti-fungicides on mouse oocyte development]
1College of Biological Sciences, China Agricultural University, Beijing 100094.
Aim:
To study the mechanism of the effect of gonadotropin-induced oocyte maturation.
Methods:
Mouse oocytes were cultured in HX-medium, and the effects of amphotericin B and ketoconazole on resumption of meiosis of mouse oocyte were examined.
Results:
1. FSH(10-200 IU/L) induced a dose-dependent manner of oocytes maturation in CEO. A maximum increase in GVBD was observed with 25-50 IU/L FSH. 2. Amphotericin tericim B (0.025-2.5 micrograms/L) caused significant increases in GVBD in CEO, which mimicked the function of FSH. 3. Ketoconazole (10(-7)-10(-3) mol/L) inhibited the effect of FSH on resumption of meiosis, but no effect on oocyte spontaneous maturation.
Conclusion:
Amphotericin B and ketoconazole are able to affect mouse oocyte maturation, and indicates that they have a regulation on FSH-induced synthesis of meiosis-activating sterol.
Insights
Follicle-stimulating hormone (FSH) and amphotericin B promote mouse oocyte maturation. Ketoconazole inhibits FSH-induced maturation, suggesting these compounds regulate meiosis-activating sterols.
Area of Science:
- Reproductive biology
- Cellular signaling
Context:
- Oocyte maturation is a critical process in female reproduction.
- Gonadotropins, like FSH, play a key role in regulating oocyte maturation.
- The precise molecular mechanisms underlying gonadotropin-induced oocyte maturation require further elucidation.
Purpose:
- To investigate the mechanism of gonadotropin-induced oocyte maturation.
- To examine the effects of amphotericin B and ketoconazole on mouse oocyte meiosis resumption.
Summary:
- Follicle-stimulating hormone (FSH) induced oocyte maturation in a dose-dependent manner.
- Amphotericin B mimicked the function of FSH, significantly increasing germinal vesicle breakdown (GVBD).
- Ketoconazole inhibited FSH-induced meiosis resumption but did not affect spontaneous maturation, indicating a regulatory role in FSH-dependent pathways.
Impact:
- This study reveals that amphotericin B and ketoconazole influence mouse oocyte maturation.
- The findings suggest these compounds modulate the synthesis of meiosis-activating sterols involved in FSH-induced maturation.
- Provides insights into the signaling pathways regulating oocyte meiotic progression.