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Related Experiment Video

Updated: Feb 1, 2026

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A genomic perspective on membrane compartment organization.

J B Bock1, H T Matern, A A Peden

  • 1Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University School of Medicine, California 94305-5426, USA.

Nature
|March 10, 2001
PubMed
Summary
This summary is machine-generated.

Researchers studied protein families involved in membrane organization across eukaryotes. The Rab family expanded significantly from yeast to humans, with SNARE proteins resolving into four distinct subfamilies in humans.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Cell Biology

Background:

  • Eukaryotic genome sequencing provides opportunities to study protein family evolution.
  • Understanding membrane compartment organization is crucial for cell biology.

Purpose of the Study:

  • To investigate the evolutionary expansion of key protein families involved in membrane organization across diverse eukaryotic genomes.
  • To identify and classify members of the SNARE, coat complex, Rab, and Sec1 protein families.

Main Methods:

  • Comparative genomics analysis of four eukaryotic genomes (yeast, worm, fly, human).
  • Identification and characterization of protein family members.
  • Phylogenetic analysis to resolve protein subfamilies.

Main Results:

  • The Rab protein family showed consistent expansion from yeast to multicellular organisms.
  • All studied protein families, particularly SNAREs, Rabs, and coat complex subunits, were significantly expanded in humans.
  • The SNARE protein family was resolved into four distinct subfamilies.

Conclusions:

  • Protein family expansion, especially Rabs and SNAREs, is a key evolutionary mechanism shaping eukaryotic membrane organization.
  • Human cells possess a highly expanded repertoire of proteins involved in membrane trafficking and compartment organization.