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Cell cycle-related changes in regulatory volume decrease and volume-sensitive chloride conductance in mouse
P Doroshenko1, V Sabanov, N Doroshenko
1Loeb Health Research Institute, Ottawa Hospital, University of Ottawa, 75 Parkdale Avenue, Ottawa, Ontario, Canada K1Y 4E9. pdoroshenko@lri.ca
Journal of Cellular Physiology
|March 10, 2001
Summary
Mouse fibroblasts can regulate cell volume after swelling, but this ability, known as regulatory volume decrease (RVD), is reduced in cells nearing mitosis. This is linked to decreased volume-sensitive chloride conductance (G(Cl,vol)).
Area of Science:
- Cell Biology
- Physiology
Background:
- Cell volume regulation is crucial for cellular function.
- Osmotic stress affects cell volume, necessitating adaptive responses like regulatory volume decrease (RVD).
Purpose of the Study:
- To investigate how cell cycle progression influences the capacity for RVD in mouse fibroblasts.
- To determine the role of volume-sensitive chloride conductance (G(Cl,vol)) in cell cycle-dependent RVD.
Main Methods:
- Utilized videomicroscopy and whole-cell patch clamp techniques.
- Studied proliferating, cell cycle-arrested (G1, G1/S, S, M phases), and serum-starved (G0) mouse fibroblasts.
- Employed pharmacological agents (e.g., mevastatin, taxol) to achieve cell cycle arrest.
Main Results:
- All studied cells exhibited RVD, but rates were lowest in cells approaching mitosis.
- Volume-sensitive chloride conductance (G(Cl,vol)) density was reduced by half in cells nearing mitosis and in newly formed daughter cells.
- Serum-starved cells (G0) showed the most significant impairment in RVD and the lowest G(Cl,vol) density.
Conclusions:
- Mouse fibroblasts generally maintain RVD capacity throughout the cell cycle.
- Mitosis represents a phase where RVD ability is compromised, correlating with reduced G(Cl,vol).
- Cell cycle status significantly impacts the efficiency of cell volume regulation.