Related Experiment Video
Updated: Aug 10, 2026
Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
Published on: March 25, 2016
[Retinoic acid inducing Fas expression in HL-60 cells]
1Department of Hematology, Beijing Tongren Hospital, Capital University of Medical Sciences, Beijing.
Objective:
To investigate the effect of all-trans retinoic acid (ATRA) and 9-cis retinoic acid (9-cis RA) on the expression of Fas and the sensitivity to anti-Fas induced apoptosis in HL-60 cells.
Methods:
Fas expression was detected by flow cytometry. Anti-Fas induced apoptosis (AFIA) was determined by morphological features, DNA fragment electrophoresis, and flow cytometric cell cycle analysis.
Results:
Fas antigen was weakly expressed on HL-60 cells. After incubation of HL-60 cells with 10(-6) mol/L ATRA or 9-cis RA for 4 days, both the Fas expression and AFIA were significantly enhanced. 9-cis RA was more potent than ATRA in increasing the sensitivity to AFIA.
Conclusion:
RA might upregulate Fas expression. Further elucidation of its molecular mechanisms might provide a rationale for new therapeutic strategies of malignant diseases.
More Related Videos
11:42Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
Published on: November 4, 2019
08:54Monitoring Leucine-Rich Repeat Containing 8 Channel (LRRC8/VRAC) Activity Using Sensitized-Emission Förster Resonance Energy Transfer (SE-FRET)
Published on: August 9, 2024