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Updated: Aug 6, 2026
Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
Published on: April 6, 2017
[PTA1 monoclonal antibody induces human platelet aggregation and intra-cytoplasmic Ca2+ elevation]
Objective:
To explore the mechanism of PTA1 monoclonal antibody (McAb) induced human platelet aggregation and its effect on intra-cytoplasmic Ca2+ level.
Methods:
Platelet aggregation, ATP releasing assay and Pollock's test were used.
Results:
PTA1 McAb induced human platelet aggregation in vitro, which could be completely inhibited by EGTA and PGI2. F(ab')2 of PTA1 McAb had no effect on CD9 or CD41 induced platelet activation and aggregation. PTA1 McAb enhanced platelet intra-cytoplasmic Ca2+ elevation.
Conclusion:
PTA1 McAb inducing platelet aggregation is related to platelet Fc receptor and CD41/CD61 (II b/III a) complex, and the induced platelet intra-cytoplasmic Ca2+ elevation is resulted from Ca2+ influx and releasing of intracytoplasmic Ca2+ storage.
