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[The characteristics and significance of oncogenes expression in oval cells cultivated in vitro]
1Department of Pathology, Sun Yat-Sen University of Medical Sciences, Guangzhou 510089.
Objective:
To study the relationship between expression characteristics of oncogenes and transformation of the oval cells cultivated in vitro.
Methods:
Liver oval cells of SD rats induced by feeding with chemical carcinogen 3'-Me-DAB for four weeks were isolated using Percoll density gradient centrifugation. The cells were continuously cultivated in vitro and the dynamic characteristics of Ha-ras, Ki-ras and c-myc genes expression in the oval cells were detected using RNA-DNA slot blot hybridization.
Results:
During long term cultivation, the population doubling time of the cells became shorter and the percentage of aneuploidy chromosomes and colonies grown in soft agar increased. Ha-ras Ki-ras and c-myc were synchronically expressed in the oval cells from different passages.
Conclusion:
The activation and synergistic over-expression of Ha-ras Ki-ras and c-myc had an important role in the proliferation and transformation of oval cells in vitro.
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