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[Detection of T-cell receptor gamma chain gene rearrangement in midline T-cell lymphomas by PCR method]
1Department of Pathology, West China University of Medical Sciences, Chengdu 610041.
Objective:
To investigate the clonality of midline T-cell lymphoma.
Methods:
Using polymerase chain reaction method and a panel of family specific primers for the variable regions of TCR-gamma gene, and totally 11 cases (22 samples) of midline T-cell lymphoma (midline malignant reticulosis) were studied.
Results:
Family specific monoclonal TCR-gamma gene rearrangements were found in 21 out of 22 samples (94.45%). There was no change of clonality during the course of disease.
Conclusion:
There is monoclonal T-cell proliferation in midline T-cell lymphoma and the clonality is not transformed with the advance of the disease. This finding provides an evidence to support the hypothesis of monoclonal origin of neoplasm.
