Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changes of nuclear structure induced by increasing temperatures.

L Vergani1, G Mascetti, C Nicolini

  • 1Department of Biophysical Sciences and Technologies M & O, School of Medicine, University of Genova, Italy. vergani@ibf.unige.it

Journal of Biomolecular Structure & Dynamics
|March 14, 2001
PubMed
Summary

This study reveals that altering nuclear structures and volume, using temperature gradients, significantly impacts chromatin organization and condensation in interphase nuclei. Nuclear volume inversely correlates with chromatin condensation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Radiation measurements in the International Space Station, Columbus module, in 2020-2022 with the LIDAL detector.

Life sciences in space research·2023
Same author

Dataset of night-time emissions of the Earth in the near UV range (290-430 nm), with 6.3 km resolution in the latitude range -51.6<L<+51.6 degrees, acquired on board the International Space Station with the Mini-EUSO detector.

Data in brief·2023
Same author

Bending analysis of sandwich panel composite with a re-entrant lattice core using zig-zag theory.

Scientific reports·2022
Same author

The NATO project: nanoparticle-based countermeasures for microgravity-induced osteoporosis.

Scientific reports·2019
Same author

Bone strain index reproducibility and soft tissue thickness influence: a dual x-ray photon absorptiometry phantom study.

European radiology experimental·2019
Same author

First Report of Johnsongrass mosaic virus (JGMV) Infecting Pennisetum purpureum in Brazil.

Plant disease·2019

Area of Science:

  • Cell Biology
  • Biophysics
  • Genomics

Background:

  • Understanding the higher-order structure of chromatin fibers is advancing.
  • The organization of interphase chromosomes within specific nuclear domains is a recent and debated area of research.

Purpose of the Study:

  • To investigate the structure and organization of chromatin in the interphase nucleus.
  • To explore the effects of temperature-induced nuclear volume changes on chromatin condensation.

Main Methods:

  • Utilized an integrated approach with complementary techniques.
  • Analyzed native CHO-K1 cells subjected to progressive heating (310 K to 410 K).
  • Employed cytometric, calorimetric, and fluorescence microscopy techniques for in situ analysis.

Related Experiment Videos

Main Results:

  • Observed significant effects of nuclear structure changes (envelope and matrix) on chromatin organization and condensation.
  • Demonstrated significant alterations in chromatin structure when nuclear volume was modified by temperature gradients.
  • Found data supporting an inverse relationship between nuclear volume and chromatin condensation.

Conclusions:

  • Nuclear envelope and matrix play crucial roles in regulating chromatin organization and condensation.
  • External forces that alter nuclear volume directly impact chromatin structure.
  • The study supports the hypothesis that increased nuclear volume leads to decreased chromatin condensation.