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Updated: Jul 24, 2026

Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging
Published on: January 2, 2014
[Use of atomic force microscopy to study the morphology and structure of viruses]
V P Polishchuk1, T P Tyvonchuk, Ie V Rengevich
1Taras Shevchenko Kyiv National University, 64 Vladimirskaya St., Kyiv, 01033, Ukraine.
Abstract:
The paper describes AFM methodic approaches in the investigation of viruses (TMV, phage T4). A possibility is shown to use this method to obtain high-quality three-dimensional image of objects without additional treatment with salts of heavy metals. The phage T4 was also a model to study the object density, using the phase shift of the AFM. Such researches can determine the internal structure of a virion and folding of nucleic acid inside a virion.
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