Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Analysis of cellular adhesion by microarray expression profiling.

V Brenner1, K Lindauer, A Parkar

  • 1Aventis Pharmaceuticals, Rainham Road South, Dagenham, Essex RM10 7XS, UK.

Journal of Immunological Methods
|March 17, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coping Strategies of Patients With Advanced Lung or Colorectal Cancer Over Time: Insights From the International ACTION Study.

Psycho-oncology·2024
Same author

Does the wearing of masks change voice and speech parameters?

European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·2021
Same author

Outcomes of femoral de-rotation osteotomy for treatment of femoroacetabular impingement in adults with decreased femoral anteversion.

Journal of hip preservation surgery·2021
Same author

Assessment of quality of life of the patients with diabetic retinopathy using National Eye Institute Visual Functioning Questionnaire (VFQ-25).

Journal of healthcare quality research·2021
Same author

Association between imaging response and survival following neoadjuvant chemotherapy in patients with resectable colorectal liver metastases: A cohort study.

Journal of surgical oncology·2021
Same author

Biotin immunoassay interference: A UK-based prevalence study.

Annals of clinical biochemistry·2020
Same journal

Optimized intracellular flow cytometry panel enables CD4 and CD8 T cell cytokine profiling in Syrian hamsters.

Journal of immunological methods·2026
Same journal

Isosulfan blue sentinel lymph node biopsy enables reliable lymph node harvest and multicolor flow cytometry in mice.

Journal of immunological methods·2026
Same journal

Type-specific antibody detection of herpes simplex virus types 1&2 (HSV-1&2) in fingerstick blood at point-of-care sites by a rapid and sensitive lateral flow immunochromatographic assay.

Journal of immunological methods·2026
Same journal

Development of chimeric DGP-IgG antibodies as quality control for celiac disease diagnosis.

Journal of immunological methods·2026
Same journal

Stepwise single-cell-resolved deep immunophenotyping pipeline to characterise immune heterogeneity and functionality in health and disease.

Journal of immunological methods·2026
Same journal

Performance evaluation of Meso Scale Discovery (MSD) quantitative serological assays for detection of binding (IgG, IgA, IgM) and ACE2 inhibitory antibody levels for SARS-CoV-2.

Journal of immunological methods·2026
See all related articles

Researchers developed a microarray platform to study gene expression in pathological conditions, especially inflammation. They identified differentially expressed genes in adhered versus non-adhered eosinophil cells, offering potential drug targets.

Area of Science:

  • Molecular Biology
  • Genomics
  • Immunology

Background:

  • Microarrays enable simultaneous analysis of numerous gene expression profiles.
  • Identifying novel genes is crucial for understanding pathological conditions, particularly those involving inflammation.
  • Eosinophils play a role in inflammatory processes, making their gene expression a key area of study.

Purpose of the Study:

  • To establish a microarray platform for identifying genes in pathological conditions with an inflammation component.
  • To investigate cell adhesion in an eosinophil model cell line (AML14.3D10).
  • To analyze the transcription profile of adhered versus non-adhered eosinophil cells.

Main Methods:

  • Utilized a microarray platform based on novel sequences from an eosinophil sequencing project.

Related Experiment Videos

  • Employed an eosinophil model cell line (AML14.3D10) to study cell adhesion.
  • Compared transcription profiles of adhered and non-adhered cells, assessing reproducibility.

    • Main Results:

    • Established a technically and biologically reproducible transcription profile for adhered and non-adhered AML14.3D10 cells.
    • Identified several genes with differential expression between adhered and non-adhered cell populations.
    • Found that differentially expressed genes in adhered cells showed restricted expression in certain tissues but were not exclusively eosinophil-specific.

    Conclusions:

    • Transcription profiling provides a robust method for analyzing gene expression in pathological contexts.
    • Differential gene expression in eosinophil adhesion offers insights into inflammatory mechanisms.
    • The identified differentially expressed genes may serve as potential targets for drug intervention in inflammatory diseases.