Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene expression studies using microarrays.

J K Burgess1

  • 1Respiratory Research Group, Department of Pharmacology, University of Sydney, New South Wales, Australia. janette@pharmacol.usyd.edu.au

Clinical and Experimental Pharmacology & Physiology
|March 17, 2001
PubMed
Summary

Advancements in gene sequencing necessitate new methods for gene analysis. Microarray technology enables the study of large gene sets, revealing gene interactions and aiding drug development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Stress of Lung Aging: Endoplasmic Reticulum and Senescence Tête-à-Tête.

Physiology (Bethesda, Md.)·2021
Same author

Human lung extracellular matrix hydrogels resemble the stiffness and viscoelasticity of native lung tissue.

American journal of physiology. Lung cellular and molecular physiology·2020
Same author

Phenotype and Functional Features of Human Telomerase Reverse Transcriptase Immortalized Human Airway Smooth Muscle Cells from Asthmatic and Non-Asthmatic Donors.

Scientific reports·2018
Same author

Latrophilin receptors: novel bronchodilator targets in asthma.

Thorax·2016
Same author

Beyond TGFβ--novel ways to target airway and parenchymal fibrosis.

Pulmonary pharmacology & therapeutics·2014
Same author

Doxycycline inhibits matrix metalloproteinase-2 secretion from TSC2-null mouse embryonic fibroblasts and lymphangioleiomyomatosis cells.

British journal of pharmacology·2011

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Genome sequencing programs are rapidly advancing, generating vast amounts of data.
  • Understanding gene function and interactions is the next frontier in biological research.
  • New techniques are needed to analyze large sets of genes simultaneously.

Purpose of the Study:

  • To introduce microarray technology as a method for gene expression analysis.
  • To explain how microarrays can help identify genes with unknown functions.
  • To highlight the potential of gene expression data in developing targeted therapies.

Main Methods:

  • Microarrays are created by spotting cDNA or oligonucleotides onto glass slides.
  • RNA from control and experimental cell populations is labeled with different fluorochromes.
  • Labeled RNA is hybridized to the microarray, and fluorescence intensity is scanned.

Main Results:

  • Data analysis software clusters genes with similar expression patterns.
  • This provides insights into gene function and interactions.
  • Identifies genes involved in biological processes and disease states.

Conclusions:

  • Microarray technology facilitates the analysis of gene expression on a large scale.
  • Understanding gene interactions enhances knowledge of molecular control.
  • This knowledge supports the development of novel drugs and gene therapies.

Related Experiment Videos