A novel method for the follow-up of shape changes in erythrocyte and other particles

The absorbance of a suspension of asymmetrical particles fluctuates when the suspension is stirred in a circular motion. The effect is due to the fact that particles move in spiral orbits and present alternatively their short or long axis to the narrow beam of light used for measurement. Changes in the shape of erythrocytes such as flattening and swelling could be detected and quantitated by measuring the change in the amplitude of the fluctuating absorbance. The diagnostic value of this method is under investigation.