[Determination of purine derivatives in blood and plasma fractions]

A method is described for the separation of adenine and guanosine from whole blood and plasma fractions, containing purinderivatives as anticoagulant-additives. The highest concentration determined per gram human albumin or gamma-globulin was 0.5 mg. Due to interactions with blood cells guanosine could only be detected in plasma which was stored for more than one week as whole blood. The amount of adenine and guanosine to be expected in therapeutical products is uncritical according to published pharmacological investigations.