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Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
06:46

Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

Published on: June 30, 2016

Granulocyte macrophage-colony-stimulating factor mRNA is stabilized in airway eosinophils and peripheral blood

S Esnault1, J S Malter

  • 1Department of Pathology, University of Wisconsin Medical School, Madison, WI 53792, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|March 20, 2001
PubMed

Insights

Airway eosinophils survive longer due to sustained granulocyte-macrophage colony-stimulating factor (GM-CSF) mRNA. Tumor necrosis factor-alpha and fibronectin enhance eosinophil survival by stabilizing GM-CSF mRNA.

Area of Science:

  • Immunology
  • Respiratory Medicine
  • Cell Biology

Background:

  • Airway eosinophils exhibit extended in vitro survival compared to peripheral blood eosinophils.
  • Autocrine granulocyte-macrophage colony-stimulating factor (GM-CSF) production is implicated in enhanced eosinophil survival, but regulatory mechanisms are unclear.

Purpose of the Study:

  • To investigate the mechanisms controlling granulocyte-macrophage colony-stimulating factor (GM-CSF) production and mRNA stability in airway eosinophils.
  • To compare GM-CSF mRNA decay rates between airway eosinophils (BALEos) and peripheral blood eosinophils (PBEos).

Main Methods:

  • Compared GM-CSF mRNA decay in BALEos and PBEos.
  • Assessed the effect of neutralizing anti-GM-CSF antibodies on BALEos survival.
  • Utilized transfection studies to determine GM-CSF mRNA stability.
  • Investigated the impact of fibronectin and TNF-alpha on PBEos survival and GM-CSF mRNA stability.

Main Results:

  • BALEos demonstrated prolonged in vitro survival and expressed GM-CSF mRNA.
  • Neutralizing anti-GM-CSF antibodies significantly inhibited BALEos survival.
  • GM-CSF mRNA was 2.5 times more stable in BALEos than in PBEos.
  • Fibronectin and TNF-alpha treatment increased PBEos survival, GM-CSF mRNA expression, and mRNA stability.

Conclusions:

  • Airway eosinophil longevity is linked to increased GM-CSF mRNA stability.
  • Tumor necrosis factor-alpha and fibronectin may enhance eosinophil survival in vivo.
  • These factors likely regulate eosinophil survival by controlling GM-CSF production at a posttranscriptional level.

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