Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Collaborative study on rat sperm motion analysis using CellSoft Series 4000 semen analyzer.

Y Ban1, M Naya, T Nishimura

  • 1Safety Assessment, Banyu Pharmaceutical Co., Ltd., 810 Nishijo, Menuma-machi, Osato-gun, Saitama 360-0214, Japan.

The Journal of Toxicological Sciences
|March 21, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Influence of neutralizing antibodies to adalimumab and infliximab on the treatment of psoriasis.

The British journal of dermatology·2013
Same author

Regulation of RalA GTPase by phosphatidylinositol 3-kinase as visualized by FRET probes.

Biochemical Society transactions·2006
Same author

Successful treatment of steroid-resistant Weber-Christian disease with biliary ductopenia using cyclosporin A.

Rheumatology (Oxford, England)·2005
Same author

Cross-clade neutralizing antibody production against human immunodeficiency virus type 1 clade E and B' strains by recombinant Mycobacterium bovis BCG-based candidate vaccine.

Vaccine·2001
Same author

Evidence of primary beta-cell destruction by T-cells and beta-cell differentiation from pancreatic ductal cells in diabetes associated with active autoimmune chronic pancreatitis.

Diabetes care·2001
Same author

Symptomatic hypothyroidism in decompensated liver cirrhosis.

Journal of clinical gastroenterology·2001
Same journal

Linalool inhalation‒induced anxiety-like effects occur independently of olfactory input and require cytochrome P450 activity.

The Journal of toxicological sciences·2026
Same journal

Protein homeostasis disruption in cisplatin-induced skeletal muscle atrophy: toxicological insights from experimental studies.

The Journal of toxicological sciences·2026
Same journal

A high-fat/high-sucrose/high-cholesterol diet leads to early indications of metabolic dysfunction-associated steatohepatitis-like lesions in obese diabetic mice.

The Journal of toxicological sciences·2026
Same journal

Sensitivity of respiratory cell lines to unsaturated carbonyl compounds in cigarette smoke is regulated by intracellular glutathione.

The Journal of toxicological sciences·2026
Same journal

Virtual internal exposures of lansoprazole administered to cytochrome P450 2C19 poor metabolizers estimated by simplified physiologically based pharmacokinetic modeling.

The Journal of toxicological sciences·2026
Same journal

A case study of virtual control group verification using historical control data at a single facility.

The Journal of toxicological sciences·2026
See all related articles

This study identified key rat sperm motion parameters for detecting compound toxicity. Percentage of motile sperm, curvilinear velocity, and head displacement are sensitive indicators of reproductive toxicity.

Area of Science:

  • Reproductive toxicology
  • Sperm analysis
  • Biomonitoring

Background:

  • Assessing the impact of chemical compounds on male reproductive health is crucial.
  • Identifying sensitive biomarkers for testicular toxicity is essential for risk assessment.
  • Standardized semen analysis can provide valuable insights into compound-induced sperm damage.

Purpose of the Study:

  • To evaluate the utility of specific rat sperm motion parameters for detecting chemical-induced reproductive toxicity.
  • To identify sensitive and reliable metrics for assessing the effects of known toxicants on sperm motility and kinematics.
  • To validate the use of the CellSoft Series 4000 semen analyzer for toxicological screening.

Main Methods:

  • Rat sperm motion parameters were analyzed using a CellSoft Series 4000 semen analyzer.

Related Experiment Videos

  • Sperm were exposed to known reproductive toxicants including alpha-chlorohydrin, boric acid, and others.
  • Changes in parameters such as % motile, VCL, ALH, and BCF were measured and compared across treatments.

    • Main Results:

    • All tested compounds decreased the percentage of motile sperm (% motile).
    • Curvilinear velocity (VCL) and amplitude of lateral head displacement (ALH) were reduced by most compounds.
    • Beat cross frequency (BCF) showed variable responses, increasing with sulfasalazine exposure.

    Conclusions:

    • Percentage of motile sperm (% motile), VCL, ALH max, and ALH mean are sensitive parameters for evaluating compound effects on sperm motion.
    • BCF can be useful for detecting specific compound-induced sperm motion alterations.
    • Linearity and circular swimming parameters were found to be less sensitive indicators of toxicity.