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Concentration of progenitor cells collected from bone marrow fluid using a continuous flow cell separator
1Department of Internal Medicine, Nara Hospital, Kinki University School of Medicine, Ikoma-city, Japan.
Summary
Red blood cells are removed from bone marrow for ABO-incompatible transplants using a Cobe Spectra cell separator. This method effectively removes red cells, preserving vital progenitor cells for successful hemopoiesis recovery.
Area of Science:
- Hematology
- Transplantation immunology
Background:
- ABO major incompatibility in bone marrow transplantation (BMT) necessitates red blood cell removal from collected marrow to prevent hemolysis.
- Failure to remove red cells can lead to transfusion reactions and compromise graft function.
Purpose of the Study:
- To evaluate the efficacy of a continuous flow cell separator (Cobe Spectra) for concentrating progenitor cells while removing red blood cells in the context of ABO-incompatible BMT.
- To assess the recovery rates of various hematopoietic progenitor cell populations and the safety of the procedure.
Main Methods:
- Bone marrow was collected and processed using a Cobe Spectra continuous flow cell separator.
- The volume of concentrated bone marrow and residual red cells was measured.
- Recovery rates for total nucleated cells, mononuclear cells, CD34+ cells, and colony-forming unit granulocyte-macrophage (CFU-GM) were determined.
- Eighteen patients undergoing allogeneic BMT received the processed marrow, and clinical outcomes regarding fever and hemolysis were monitored.
Main Results:
- The average volume of concentrated bone marrow was 132 +/- 47 ml, with an average of 5.1 +/- 2.4 ml of red cells remaining.
- An effective red cell removal rate of 97.6% was achieved.
- High recovery rates were observed for progenitor cells: 40.6% for total nucleated cells, 77.9% for mononuclear cells, 100% for CD34+ cells, and 93.9% for CFU-GM.
- No signs of fever or hemolysis were reported in 18 patients during the transfusion of concentrated marrow.
Conclusions:
- The Cobe Spectra effectively concentrates progenitor cells and removes red blood cells, achieving high recovery rates for critical hematopoietic stem cell populations.
- This technique is safe and applicable for ABO-incompatible bone marrow transplantation and for processing cryopreserved stem cells.
- The high recovery of progenitor cells supports adequate hemopoiesis restoration post-transplant.