Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Particle electrophoresis for quality assurance and process control.

G V Seaman1, R J Knox

  • 1Western Biomedical Research Institute, Eugene, OR 97402, USA.

Electrophoresis
|March 22, 2001
PubMed
Summary

Analytical Particle Electrophoresis (APE) offers robust process control for life sciences. This method measures particle surface charge to ensure manufacturing quality and optimize product development, enhancing consistency.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Automated particle electrophoresis:  modeling and control of adverse chamber surface properties.

Analytical chemistry·2011
Same author

Reduction of mitomycin C is catalysed by human recombinant NRH:quinone oxidoreductase 2 using reduced nicotinamide adenine dinucleotide as an electron donating co-factor.

British journal of cancer·2006
Same author

Targeting enzymes to cancers--new developments.

Expert opinion on investigational drugs·2005
Same author

Aerobic nitroreduction by flavoproteins: enzyme structure, mechanisms and role in cancer chemotherapy.

Mini reviews in medicinal chemistry·2002
Same author

Telomerase-specific suicide gene therapy vectors expressing bacterial nitroreductase sensitize human cancer cells to the pro-drug CB1954.

Oncogene·2001
Same author

Gene-directed enzyme prodrug therapy (GDEPT)--recognizing the present limitations of gene therapy for the treatment of cancer.

Current opinion in investigational drugs (London, England : 2000)·2001

Area of Science:

  • Life Sciences
  • Analytical Chemistry
  • Process Engineering

Background:

  • Life science companies globally seek ISO and GMP compliance for manufacturing and product development.
  • Process control is crucial for ensuring product quality and consistency in life science industries.
  • Analytical Particle Electrophoresis (APE) is emerging as a valuable tool for process development and control.

Purpose of the Study:

  • To explore the application of Analytical Particle Electrophoresis (APE) in life science process control.
  • To demonstrate the utility of APE for assessing process consistency of both particles and the manufacturing environment.
  • To highlight the role of polymer microspheres as control particles for instrument validation and assay medium probing.

Main Methods:

  • Utilizing Analytical Particle Electrophoresis (APE) to measure surface charge properties of small particles (>100 nm).

Related Experiment Videos

  • Employing direct measurements of charge properties of process particles and "reporter" particles for optimization.
  • Leveraging advanced instrumentation and certified control particle preparations (e.g., NIST) for method validation.

    • Main Results:

    • APE provides flexible electrophoretic strategies for assessing process consistency.
    • Surface charge measurements offer insights into the behavior of colloidal particles in manufacturing processes.
    • Polymer microspheres serve effectively as control particles for instrument validation and as probes for assay media.

    Conclusions:

    • APE is a powerful technique for enhancing process control and quality assurance in life science manufacturing.
    • Understanding and measuring particle surface charge is key to optimizing life science products and processes.
    • The integration of APE with validated control particles strengthens its applicability in regulated environments.