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Particle diameter influences adhesion under flow
V R Shinde Patil1, C J Campbell, Y H Yun
1The Department of Chemical Engineering, Ohio University, Athens 45701, USA.
Biophysical Journal
|March 22, 2001
Summary
Cell size significantly impacts how cells attach, roll, and adhere to blood vessels. Larger cells require less shear stress to move and roll faster, confirming theoretical models on cell diameter
Area of Science:
- Biophysics
- Cellular Biology
- Biomaterials
Background:
- Circulating cell diameters range from 2 to 20 micrometers, influencing interactions with vascular endothelium.
- Mathematical models suggest cell diameter is crucial for adhesion, but experimental validation is limited.
Purpose of the Study:
- To experimentally investigate the role of cell diameter in cell adhesion to the vascular endothelium.
- To compare the adhesion dynamics of microspheres with varying diameters coated with P-selectin glycoprotein ligand-1 (PSGL-1).
Main Methods:
- Microspheres of 5-, 10-, 15-, and 20-micrometer diameters were coated with the recombinant PSGL-1 construct 19.ek.Fc.
- In vitro flow conditions were used to compare the adhesion of these microspheres to P-selectin.
- Attachment rates, shear stress for detachment, and rolling velocity were measured.
Main Results:
- At high shear, attachment rate decreased with increasing microsphere diameter; at low shear, diameter had no effect.
- Shear stress required to detach firmly adhered microspheres decreased as diameter increased.
- Rolling velocity of microspheres increased with increasing diameter.
Conclusions:
- Cell attachment, rolling, and firm adhesion are functions of particle diameter.
- Experimental results provide evidence supporting theoretical models on the significance of cell diameter in adhesion dynamics.