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Updated: Jul 16, 2026
19:16
The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
Published on: March 18, 2010
Factors affecting short-term and long-term stabilities of proteins
T Arakawa1, S J Prestrelski, W C Kenney
1Amgen Inc., Amgen Center, Thousand Oaks, CA, USA. tarakawa2@aol.com
Advanced Drug Delivery Reviews
|March 22, 2001
Summary
Proteins are unstable and easily damaged. This review explores how additives protect proteins from stress and ensure long-term stability for storage.
Area of Science:
- Biochemistry
- Protein chemistry
- Physical chemistry
Background:
- Proteins exhibit marginal stability, making them susceptible to denaturation.
- Environmental stresses (solution, frozen, dried states) can compromise protein integrity.
Purpose of the Study:
- To review mechanisms of additive-mediated protein stabilization.
- To discuss factors critical for long-term protein storage.
Main Methods:
- Literature review of protein stabilization mechanisms.
- Analysis of additive effects on protein stability.
Main Results:
- Additives can mitigate protein damage caused by various stresses.
- Understanding stabilization mechanisms is key to preventing denaturation.
Conclusions:
- Additives are crucial for preserving protein structure and function.
- Careful consideration of storage conditions and additives ensures protein longevity.
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