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[Effect of adrenomedullin on bronchoconstriction responses induced by histamine]
Objective:
To observe the effect of synthetic human adrenomedullin (AM) on anesthetized guinea pigs bronchoconstriction induced by histamine.
Method:
The guinea pigs were divided into saline group; isoproterenol groups at the dosages of 0.03 microgram/kg, 0.3 microgram/kg, 3 micrograms/kg and 30 micrograms/kg; adrenomedullin groups at the dosages of 0.02 microgram/kg, 0.2 microgram/kg, 2 micrograms/kg and 20 micrograms/kg. There were seven guinea pigs a group. Guinea pigs were anesthetized by sodium pentobarbital, normal saline group, isoproterenol and adrenomedullin at different dose i.v., while histamine (10(-4) mol/L) were vasolated and delivered into the airway. Body plethysmogroph was used to measure airway resistance and lung compliance.
Result:
The increment of airway resistance and decrement of lung compliance induced by histamine were inhibited significantly by isoproterenol at the dosages of 0.3 microgram/kg, 3 micrograms/kg and 30 micrograms/kg (P < 0.05). The increase of airway resistance was inhibited significantly by adrenomedullin at the dosages of 2 micrograms/kg and 20 micrograms/kg (P < 0.05). The decrease of lung compliance induced by histamine was inhibited significantly by adrenomedullin at 0.2 microgram/kg, 2 micrograms/kg and 20 micrograms/kg (P < 0.05).
Conclusion:
Adrenomedullin inhibited the bronchoconstriction induced by histamine significantly.