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Related Experiment Videos

Audit and internal quality control in immunohistochemistry.

P Maxwell1, W G McCluggage

  • 1Quantitative Biomarkers Group, Cancer Research Centre, Queens University of Belfast and Institute of Pathology, Royal Group of Hospitals Trust, Belfast BT12 6BA, Northern Ireland, UK. p.maxwell@pub.ac.uk

Journal of Clinical Pathology
|March 27, 2001
PubMed
Summary

This study introduces a scoring system for internal quality control in immunohistochemistry. The system helps assess staining quality and identify problematic antibodies like neurone specific enolase (NSE).

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Training in molecular cytopathology testing.

Cytopathology : official journal of the British Society for Clinical Cytology·2017

Area of Science:

  • Surgical Pathology
  • Immunohistochemistry Quality Control
  • Diagnostic Cytology

Background:

  • Internal quality control (IQC) procedures for immunohistochemistry (IHC) are not consistently described.
  • Standardized methods are needed to evaluate the reliability of IHC staining in surgical pathology.
  • Existing positive and negative controls require a systematic approach for effective interpretation.

Purpose of the Study:

  • To describe a novel method for internal quality control in immunohistochemistry.
  • To introduce a scoring system for objective assessment of IHC slide quality.
  • To evaluate the performance of frequently used antibodies using the proposed scoring system.

Main Methods:

  • A retrospective audit of IHC cases and positive tissue controls over three years (1996-1998).

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  • Evaluation of 10 frequently used antibodies, focusing on test cases and monthly positive controls.
  • Development of a scoring system (0-8) assessing staining intensity, uniformity, specificity, background, and counterstaining.

    • Main Results:

    • The scoring system effectively classified staining as optimal (7-8), borderline (5-6), or unacceptable (0-4).
    • Most antibodies demonstrated optimal or borderline staining quality.
    • Neurone specific enolase (NSE) showed frequent unacceptable or borderline staining due to low intensity, poor specificity, and high background.

    Conclusions:

    • The developed scoring system provides a reliable method for assessing immunohistochemically stained slides.
    • Routine implementation of such a scoring system is crucial for everyday internal quality control in pathology labs.
    • Identification and potential exclusion of antibodies with consistently poor staining (e.g., NSE) improve diagnostic accuracy.