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Related Experiment Videos

Autoreactive isotype-specific T cells determine B cell frequency.

F Granucci1, F P Di Tota, G Raimondi

  • 1CNR, Cellular and Molecular Pharmacology Center, Milan, Italy.

European Journal of Immunology
|March 27, 2001
PubMed
Summary
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Naturally occurring T cells suppress immunoglobulin G2a (IgG2ab) levels in mice. These findings reveal a novel mechanism for regulating antibody production and maintaining immune homeostasis.

Area of Science:

  • Immunology
  • T cell biology
  • B cell regulation

Background:

  • T cell-B cell interactions are crucial for immune homeostasis.
  • Negative control of B cells by T cells is established, but the role of endogenous anti-IgG2ab T cells in Ighb mice is unknown.
  • Previous studies generated anti-IgG2ab TCR-transgenic mice, demonstrating chronic IgG2ab suppression.

Purpose of the Study:

  • To investigate the role of endogenously matured anti-IgG2ab T cells in controlling IgG2ab+ B cell function in Ighb mice.
  • To determine if T cells expressing anti-IgG2ab T cell receptor (TCR) can be positively selected and negatively control IgG2ab serum levels.
  • To explore the presence and function of naturally occurring anti-IgG2ab T cells in Ighb mice.

Main Methods:

  • Generation and analysis of anti-IgG2ab TCR-transgenic mice.

Related Experiment Videos

  • Assessment of T cell selection in the thymus.
  • Quantification of peripheral T cell frequency and IgG2ab serum levels.
  • Analysis of single TCR chain transgenic mice.
  • Isolation and characterization of T cells from non-transgenic Ighb mice.

    • Main Results:

    • T cells with high-density anti-IgG2ab TCR expression were positively selected in the thymus of IgG2ab-expressing animals.
    • These T cells migrated to peripheral lymphoid organs and suppressed IgG2ab serum levels.
    • Single TCR chain transgenic T cells also reached the periphery and suppressed IgG2ab.
    • Serum IgG2ab concentration inversely correlated with the frequency of peripheral Ig-specific T cells.
    • Anti-IgG2ab T cells were found in the periphery of non-transgenic Ighb mice.

    Conclusions:

    • T cells expressing anti-IgG2ab TCR are naturally selected and functional in Ighb mice.
    • These T cells negatively regulate IgG2ab serum levels.
    • Anti-IgG2ab T cells are endogenously present and contribute to IgG2ab homeostasis in Ighb mice.