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Effects of changrolin on potassium currents in guinea pig and rabbit single heart cells
1Department of Physiology, College of Medicine, Xiamen University, Xiamen 361005, China.
Aim:
To elucidate whether or not changrolin (CRL) modifies the potassium currents (ITO, IK, and IKl) in myocardial cells.
Methods:
A tight seal whole-cell patch-clamp technique was used to record ITO, IK, and IKl in single cells isolated from guinea pig and rabbit hearts.
Results:
At a clinically relevant concentration, CRL 50 mumol.L-1 inhibited the transient outward current (ITO) by 17.7% +/- 2.4% (n = 8) in rabbit atrial cells. The voltage-dependence of steady-state inactivation of ITO was not affected by CRL. This concentration of CRL did not influence the time-independent inward rectifier or the delayed rectifier K+ currents (IKl and IK, respectively) in rabbit and guinea pig ventricular cells.
Conclusion:
CRL inhibited ITO, but not IK nor IKl.