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Updated: Aug 13, 2026
CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells
Published on: October 18, 2014
Comet electrophoresis of blood nucleated cells in genotoxicity assessment
1State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031, China.
Aim:
Genotoxicity evaluations of several different chemicals including L-4-oxalysine, 10-Hydroxycamptothecin (HCT), 19-norprogesteron (ST1435), dimethyl sulfoxide (Me2SO), bleomycin (BLM), and mitomycin C (MMC).
Methods:
Alkaline comet assay in vitro (single cell gel) (SCG).
Results:
L-4-oxalysine and HCT did not cause directly DNA damage. ST1435, the subdermal implant progestin, had no effect on DNA damage until the dose level up to 4 mmol.L-1. Me2SO did not increase DNA damage at concentration below 2%, but showed a concentration-dependent DNA damage at > or = 4%. Bleomycin and mitomycin C demonstrated a strong dose-dependent DNA damage.
Conclusion:
Comet assay as a tool to test the genotoxicity of suspected chemicals, is rapid, simple, sensitive, good reproducible, and inexpensive.
