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Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture
Published on: October 12, 2018
Human immunodeficiency viruses types 1 and 2 infection among replacement blood donors in Mumbai (Bombay)
H A Kamat1, D D Banker, G V Koppikar
1Department of Microbiology, Topiwala National Medical College and B.Y.L., Nair Municipal Hospital, Mumbai Central, Mumbai (Bombay)-400 008. hakamat@xtra.co.nz
Abstract:
Between 1993-96, blood donated by 12,235 replacement blood donors was screened by various Enzyme Linked Immunosorbent Assays for detecting antibodies to Human Immunodeficiency Viruses types 1 and 2 according to the guidelines specified by Indian--Food and Drug Administration. 222 replacement blood donors (1.81%) were found to be seropositive for antibodies to Human Immunodeficiency Virus types 1 and 2. Furthermore, the ImmunoComb II HIV 1 & 2 BiSpot rapid sandwich ELISA in a comb format was used for differentially identifying HIV-1 and/or HIV-2 infection among these blood donors in Mumbai. Our data indicates that there is a low seroprevalence of HIV-1-2 infection among replacement blood donors in Mumbai (Bombay). Among them, while HIV-1 is still the predominant virus, dual HIV-1-2 and HIV-2 only infections are steadily increasing.
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