Related Experiment Videos
Determination of protein-protein interactions of ICIn by the yeast two-hybrid system
A Schmarda1, F Fresser, M Gschwentner
1Department of Physiology, Institute for Medical Biology and Human Genetics, University of Innsbruck, Austria.
Abstract:
ICln is a ubiquitously expressed eukaryotic protein. Expression of the protein in Xenopus laevis oocytes, the knocking-down of the protein in fibroblasts, or the reconstitution of the protein in lipid bilayer led to the assumption that this protein is an ionic channel or a significant part thereof. However, other possible roles for ICln in potential regulatory mechanisms have been postulated, as diverse as regulator of cell morphology by interacting with the Skb1 protein and/or interaction with core spliceosomal proteins. Here we show that ICln is able to interact with SnRNP core proteins SmD1, SmD2, SmD3, SmX5 and SmB/B'.