Analysis of hyaluronidase diffusion for histochemical purposes

Observations on hyaluronidase difussion from natural and artificial objects to precipitated medium are described. The conclusions from this investigation are: (1) hyaluronidase diffusing from biological objects in a precipitated medium is an active enzyme. (2) hyaluronidase diffusion may be utilized for localization of the enzyme. (3) certain parameters of the reaction hyaluronidase--hyaluronic acid may be measured with the use of the notion "diffusion radius".