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Related Experiment Videos

Pneumoperitoneum upregulates preproendothelin-1 messenger RNA.

J A Ambrose1, R P Onders, N T Stowe

  • 1Department of Surgery, Case Western Reserve University, University Hospitals of Cleveland, 11100 Euclid Avenue, Cleveland, OH 44106, USA.

Surgical Endoscopy
|April 5, 2001
PubMed
Summary

Laparoscopic procedures decrease kidney function by increasing Endothelin-1 (ET-1). This study shows ET-1 upregulation in the kidney during pneumoperitoneum, suggesting ET-1 antagonism may protect renal function.

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Area of Science:

  • Nephrology
  • Surgical Physiology
  • Molecular Biology

Background:

  • Laparoscopic pneumoperitoneum is associated with reduced glomerular filtration rate (GFR) and urine volume (UV).
  • Endothelin-1 (ET-1), a potent renal vasoconstrictor, is a potential mediator of this renal dysfunction.
  • Investigating ET-1's role is crucial for understanding and mitigating laparoscopic-induced kidney injury.

Purpose of the Study:

  • To evaluate the impact of carbon dioxide (CO2) pneumoperitoneum on renal function.
  • To determine the effect of pneumoperitoneum on ET-1 gene expression in the kidney.
  • To localize the ET-1 peptide within renal tissues subjected to laparoscopic conditions.

Main Methods:

  • Experiments were conducted on anesthetized Sprague-Dawley rats divided into three groups: pneumoperitoneum, sham operation, and normal control.

Related Experiment Videos

  • Glomerular filtration rate (GFR) and urine volume (UV) were measured before, during, and after insufflation.
  • Kidney tissue was analyzed for preproET-1 (ppET-1) mRNA levels using reverse transcription-polymerase chain reaction (RT-PCR) and for ET-1 peptide localization via immunohistochemistry (IHC).

    • Main Results:

    • Pneumoperitoneum significantly decreased GFR by 87% and UV by 79%, with values returning to baseline post-desufflation.
    • RT-PCR revealed a significant increase in ppET-1 mRNA expression in the laparoscopic group compared to control and sham groups.
    • IHC demonstrated enhanced ET-1 peptide expression in the vascular endothelium and proximal tubular cells of kidneys from the laparoscopic group.

    Conclusions:

    • Pneumoperitoneum leads to increased ET-1 gene and peptide expression in the kidney.
    • Elevated ET-1 levels, particularly in renal vasculature and proximal tubules, may contribute to observed renal dysfunction.
    • Targeting ET-1 could offer a therapeutic strategy to protect kidney function during prolonged laparoscopic procedures or in allograft transplantation.