Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Cytosensor Microphysiometer: technology and recent applications.

¹Molecular Devices GmbH, Ismaning, Germany. frank.hafner@moldev.de

Biosensors & Bioelectronics

|April 5, 2001

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Successful long-term systemic sclerosis treatment by high-frequent low-dose B cell-depleting therapy.

Journal of autoimmunity·2024

Same author

COPART Risk Score, Endothelial Dysfunction, and Arterial Hypertension are Independent Risk Factors for Mortality in Claudicants.

European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery·2016

Same author

COPART Risk Score Predicts Long-term Mortality in Peripheral Arterial Occlusive Disease.

European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery·2015

Same author

Lymphocyte-to-monocyte ratio: a novel marker for critical limb ischemia in PAOD patients.

International journal of clinical practice·2014

Same author

Acute wiiitis representing as thrombosis of the inferior vena cava and left pelvic veins.

Phlebology·2014

Same author

Safety and efficacy of periprocedural anticoagulation with enoxaparin in patients undergoing peripheral endovascular revascularization.

Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·2013

The Cytosensor Microphysiometer uses light addressable potentiometric sensors (LAPS) to measure cellular acidification, revealing cell signaling pathways. This technology provides rapid, comprehensive insights into cellular responses, reducing the need for multiple assays.

Area of Science:

Cell Biology
Biotechnology
Analytical Chemistry

Background:

Cellular energy metabolism is linked to ATP usage and acid excretion.
Perturbations in ATP levels, like receptor activation, alter acid excretion.
Proton extrusion is a common parameter in membrane-bound receptor activation.

Purpose of the Study:

To provide an overview of Cytosensor Microphysiometer technology.
To demonstrate the system's capability in measuring acidification rates.
To showcase applications in diverse cell and receptor types.

Main Methods:

Utilizes a light addressable potentiometric sensor (LAPS) to detect extracellular acidification.
Measures changes in proton extrusion as an indicator of cellular response.

Related Experiment Videos

Employs signal transduction probes to elucidate specific receptor pathways.

Main Results:

The Cytosensor Microphysiometer detects functional cellular responses within minutes.
It provides novel information on cell signaling pathways, often missed by other methods.
The system successfully measured acidification rates across various cell and receptor types.

Conclusions:

Cytosensor Microphysiometer technology offers a rapid and versatile tool for studying cell signaling.
It enables receptor pathway elucidation without prior pathway knowledge.
The system functions as a single assay, minimizing the need for multiple experimental setups.