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Related Experiment Videos

The schistosome egg: development and secretions.

P D Ashton1, R Harrop, B Shah

  • 1Department of Biology, University of York. pda2@york.ac.uk

Parasitology
|April 6, 2001
PubMed
Summary

Schistosome egg secretions, including proteases, are produced by the mature egg envelope, not the miracidium. This understanding aids research into schistosome egg migration and liver pathology.

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Area of Science:

  • Parasitology
  • Cell Biology
  • Biochemistry

Background:

  • Schistosome eggs cause significant pathology, particularly in the liver.
  • Understanding egg development and secretions is crucial for elucidating disease mechanisms.

Purpose of the Study:

  • To investigate the development of the schistosome egg envelope and its secretions.
  • To identify the source and composition of schistosome egg secretions (ESP).
  • To understand the role of ESP in egg migration and pathology.

Main Methods:

  • Electron microscopy to study egg envelope development.
  • Biosynthetic labeling and collection of egg secretions (ESP).
  • SDS-PAGE and casein substrate gel electrophoresis to analyze ESP composition and enzymatic activity.
  • Immunocytochemistry to localize ESP.

Main Results:

  • The subshell envelope develops early and contains endoplasmic reticulum in mature eggs, indicating protein synthesis.
  • Reynolds' layer, potentially accumulated secretions, appears in mature eggs.
  • Mature eggs release ESP containing 6 distinct protein bands and 2 proteases, differing from soluble egg antigens.
  • ESP is localized to the mature egg envelope.

Conclusions:

  • The mature egg envelope is the primary source of schistosome egg secretions (ESP).
  • ESP contains proteases, suggesting a role in tissue migration and pathology.
  • These findings offer insights into schistosome pathogenesis and potential therapeutic targets.

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