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Updated: Oct 8, 2026
HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells
Published on: July 20, 2016
Detection of messenger RNA in leukocytes or plasma of patients with chronic myeloid leukemia
N H Silva1, G Pimenta, W A Pulcheri
1Laboratório de Controle da Expressão Genica, Instituto de Biofísica Carlos Chagas Filho, UFRJ, Centro de Ciências da Saude (CCS), Ilha do Fundão, Rio de Janeiro, RJ, CEP 21949-900, Brazil.
Abstract:
We investigated the presence of free mRNA in the plasma of patients with chronic myeloid leukemia (CML), through RT-PCR analysis of G3PDH, a metabolism gene. We also analysed the presence of mRNA for HLM, a human oxysterol-binding protein homologue recently described as a potential marker for blood dissemination of solid tumors. Our results showed the presence of metabolism G3PDH mRNA in the plasma of 5/11 (45%) CML patients studied but HLM mRNA was not detected in any of the plasma studied. HLM mRNA was detected in the leukocytes of 4/5 (80%) CML patients. This work reports for the first time free mRNA in the plasma of CML patients. Our results also suggest that the detection of HLM could be a potential molecular marker for the follow-up in hematological malignancies.
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