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Updated: Aug 15, 2026

Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy
Published on: December 9, 2011
Digital imaging of surgical specimens using a wet scanning technique
1Department of Cellular Pathology, Epsom General Hospital, Dorking Road, Epsom, Surrey KT18 7EG, UK. matthew@sthelier.sghms.ac.uk
Aim:
To develop a simple method of recording digital images of surgical specimens on to a personal computer (PC) for use in presentations for teaching and reporting of their pathology.
Methods:
A perspex box was constructed to international A4 size 100 mm deep. This box had a base of 3 mm clear perspex with sides and top of 5 mm white perspex. This box was partially filled with distilled water and a specimen immersed in it. It was then placed on top of a standard A4 scanner. The specimen was then scanned into a PC using image capture software.
Results:
The images produced showed noticeable improvement over normal photographs, especially with specimens prone to wet highlights.
Conclusions:
The method has proved to be a rapid and efficient means of producing macroscopic images of surgical specimens.
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