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Detection of interleukin-8 in exudates from normal and inflamed human dental pulp tissues
1Department of Endodontics, School of Stomatology, Fourth Military Medical University, No. 17 ChangLe Xi Road, Xincheng District, Xi'an, Shaanxi, 710032, P. R. China.
Objective:
The purpose of this study was to investigate the level of IL-8 in exudates clinically obtained from normal and inflamed human dental pulp tissues so as to reveal the possible relationship between IL-8 and pulpitis.
Methods:
Samples of 2 microliters of pulpal exudate from each normal or clinically diagnosed as acute or chronic pulpitis teeth was obtained by filter paper strips and IL-8 level was measured by ELISA method.
Results:
No IL-8 was detected in the samples from normal pulp, but significant amount of IL-8 appeared in inflamed pulp tissues, and the level of IL-8 in exudates of acute stage of pulpitis was higher than that of chronic stage (P < 0.01).
Conclusions:
This study demonstrates that IL-8 is produced and accumulated in pulp inflammation and may play a role in the occurrence and development of human pulpitis.