Related Experiment Video

Updated: Oct 8, 2026

Reconstruct Human Retinoblastoma In Vitro
06:52

Reconstruct Human Retinoblastoma In Vitro

Published on: October 11, 2022

Abnormalities of the human Rb tumor suppressor gene in oral squamous cell carcinoma

H Huang1, J Wang, J Li

  • 1Department of Oral and Maxillofacial Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University of Medical Sciences, No. 107 Yanjiang W. Road, Guangzhou, 510120, P. R. China.

The Chinese Journal of Dental Research
|April 21, 2001
PubMed
Abstract

Insights

Retinoblastoma (Rb) gene abnormalities, including deletion and rearrangement, were found in 26.7% of oral squamous cell carcinoma (OSCC) cases. The study indicates a low rate of Rb gene alteration in OSCC.

Area of Science:

  • Oncology
  • Genetics
  • Molecular Biology

Background:

  • Oral squamous cell carcinoma (OSCC) is a significant global health concern.
  • The Retinoblastoma (Rb) tumor suppressor gene plays a crucial role in cell cycle regulation and is implicated in various cancers.

Purpose of the Study:

  • To investigate the structural status of the Retinoblastoma (Rb) gene in primary oral squamous cell carcinoma (OSCC).
  • To determine the frequency and types of Rb gene alterations in OSCC samples.

Main Methods:

  • DNA Southern blotting assay was employed to analyze the Rb gene.
  • Digoxin (Dig)-labeled Rb cDNA probe was utilized for detection.

Main Results:

  • Structural abnormalities of the Rb gene were detected in 8 out of 30 (26.7%) invasive OSCC samples.
  • Alterations included partial gene deletion (7 cases) and gene rearrangement (1 case).
  • No significant correlation was observed between Rb gene alterations and clinicopathological factors such as sex, age, tumor site, differentiation, or TNM stage.

Conclusions:

  • Abnormalities in the Rb tumor suppressor gene, specifically deletion and rearrangement, are present in OSCC.
  • The overall rate of Rb gene abnormality in this cohort of OSCC patients is low.

More Related Videos

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment07:29

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment

Published on: April 22, 2019

Related Concept Videos

The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...