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Complete nucleotide sequence of cherry necrotic rusty mottle virus
1Federal Biological Research Center for Agriculture and Forestry, Institute for Plant Protection in Fruit Crops, Dossenheim, Federal Republic of Germany.
Archives of Virology
|April 24, 2001
Summary
A new virus, cherry necrotic rusty mottle virus (CNRMV), was identified from diseased cherry trees. Its complete genome sequence reveals conserved and unique genes, offering insights into cherry virus diseases.
Area of Science:
- Plant virology
- Molecular biology
- Genomics
Background:
- Cherry necrotic rusty mottle disease affects stone fruit production.
- A virus closely related to cherry green ring mottle virus (CGRMV) was isolated from symptomatic trees.
Purpose of the Study:
- To identify and characterize the causal agent of cherry necrotic rusty mottle disease.
- To determine the complete genomic sequence of the newly isolated virus.
Main Methods:
- Virus isolation from infected cherry trees.
- Next-generation sequencing to determine the complete viral genome.
- Bioinformatic analysis of open reading frames (ORFs) and conserved domains.
Main Results:
- The isolated virus was named cherry necrotic rusty mottle virus (CNRMV).
- The complete genome sequence of CNRMV (8,432 nucleotides) was determined.
- CNRMV possesses 7 ORFs, with 5 conserved among related viruses (fovea-, allexi-, potex-, carlaviruses).
- Two unique ORFs (2a and 5a) were identified, nested within larger ORFs, showing similarity to those in CGRMV.
Conclusions:
- CNRMV is the causal agent of cherry necrotic rusty mottle disease.
- The genomic organization of CNRMV shares features with other viruses but includes unique elements.
- The function of the novel nested ORFs in CNRMV requires further investigation.