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Buffering action of human dentin in vitro
1Department of Dental Materials, UFR Odontologie, 27 Bd Jean Moulin, 13385 Marseille, France. jcamps2035@aol.com
The Journal of Adhesive Dentistry
|April 25, 2001
Summary
Dentin’s mineral content provides nearly all its buffering capacity, significantly limiting acid diffusion. This study found no difference between normal and caries-affected dentin in buffering abilities for dental acids.
Area of Science:
- Biomaterials Science
- Dental Materials
- Biomineralization
Background:
- Dentin's buffering capacity is crucial for protecting underlying pulp from acidic challenges.
- Understanding the contributions of mineral and organic phases is key to predicting dentin's response to acids in restorative dentistry.
Purpose of the Study:
- To quantify the mineral and organic phases' roles in dentin's buffering capacity.
- To compare the buffering capacity of normal versus caries-affected dentin against acids used in adhesive dentistry.
Main Methods:
- Human coronal dentin disks (normal and caries-affected) were subjected to various acids.
- Acid uptake, recovery, and diffusion across dentin were measured via titration.
- Buffering capacity was assessed using dentin powder, demineralized dentin powder, and hydroxyapatite powder.
Main Results:
- Dentin uptake was low (10-12%), with minimal diffusion (1-2%) across 0.6 mm.
- Acid concentration and application time showed limited impact on H+ diffusion.
- Normal and caries-affected dentin exhibited similar buffering capacities.
- The mineral phase accounts for almost all of dentin's buffering capacity.
Conclusions:
- Dentin's high buffering capacity, primarily from its mineral component, effectively prevents significant acid diffusion.
- These findings are relevant for understanding dentin's interaction with acids in dental procedures.