Related Experiment Videos
On-line process control of liquid chromatography
M Fransson1, A Sparén, B Lagerholm
1Analytical Development, AstraZeneca R&D Mölndal, Sweden.
Analytical Chemistry
|April 26, 2001
Summary
A new liquid chromatography process control (LCPC) system monitors chromatographic runs in real-time. This approach enhances data quality and reduces the need for replicate standards, improving analytical process control.
Area of Science:
- Analytical Chemistry
- Process Analytical Technology (PAT)
Background:
- Traditional liquid chromatography relies on repeated injections of standards for system stability assessment.
- This method is often insufficient for comprehensive real-time process monitoring.
- There is a need for enhanced quality control during analytical runs.
Purpose of the Study:
- To introduce a novel liquid chromatography process control (LCPC) system.
- To treat the analytical run as a process generating chromatographic data as its product.
- To enhance data quality through continuous monitoring of the chromatographic system.
Main Methods:
- Development of a liquid chromatography process control (LCPC) system.
- Monitoring of key parameters like column and injection valve pressure.
- Application of chemometrics for data interpretation and multivariate statistical process control (MSPC) charts.
- Utilizing Principal Component Analysis (PCA) models for dynamic injection and static elution phases.
Main Results:
- Continuous creation and upgrading of PCA models for different chromatographic phases.
- Real-time display of results on MSPC charts with prompt notification upon exceeding error limits.
- Demonstration of LCPC as a continuous system suitability test.
Conclusions:
- LCPC offers enhanced control over liquid chromatography analyses.
- The system allows for a reduction in the number of standards and replicates needed.
- Facilitates improved troubleshooting and overall analytical process efficiency.