Ultrastructural immunolocalization of enamel matrix proteins during early stages of ameloblast differentiation

Enamel matrix proteins (EMP) represent specific molecular markers of ameloblast secretion. In order to study early differentiation stages of the cells of the inner enamel epithelium, we have investigated the ultrastructural localization of EMP-immunoreactivity in rat tooth germ. Pre-secretory stages of ameloblast differentiation were identified by the absence of EMP-immunoreactivity within epithelial cells as well as adjoining extra-cellular matrix. During subsequent secretory stages EMP-like immunoreactive material could be detected both within epithelial cells as well as within the adjoining extra-cellular matrix. The intensity of the immunoreactivity increased while advancing with the differentiation of epithelial cells. Intracellularly, EMP-immunoreactivity was detectable in cytoplasmic compartments involved in exocrine secretion pathway. During the early secretory stage, EMP-immunoreactive material was also detectable in the basement membrane of the epithelial-mesenchymal interface and within the pre-dentine, close to odontoblast plasma membranes and processes. It is thus suggested that EMP may cross the basement membrane between epithelial and mesenchymal cells. Our study suggests that this aspect might be important in molecular mechanisms that regulate epithelial-mesenchymal interactions during odontogenesis.