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Updated: Oct 8, 2026

Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation
Published on: June 26, 2013
Long-term potentiation induced by nicotine in CA1 region of hippocampal slice is Ca(2+)-dependent
1Department of Pharmacology, Sun Yat-sen University of Medical Sciences, Guangzhou, 510089, China.
Aim:
To observe the effects of Ca2+ on hippocampal long-term potentiation (LTP) induced by nicotine in CA1 region of rat hippocampal slice.
Methods:
Extracellularly recorded population spikes (PS) of the pyramidal cell layer in the hippocampal CA1 region in vitro.
Results:
Nicotine 1 mumol.L-1 induced LTP in the hippocampal CA1 region. It did not induce LTP in CA1 region when CA2+ was removed from artificial cerebrospinal fluid (ACSF). Nifedipine 1 and 10 mumol.L-1 partly inhibited LTP induced by nicotine, and thapsigargin 1 and 10 mumol.L-1 completely inhibited LTP induced by nicotine.
Conclusion:
LTP induced by nicotine in hippocampal CA1 region is Ca(2+)-dependent. Both Ca2+ influx and Ca2+ release participate in the induction of LTP.
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