[Determination of vincristini sulfas in tumor cells by high performance liquid chromatography]

Vincristini sulfas (VCR) is an important common antitumor drug. The resistance to anticancer drug is the main cause of the chemotherapy failure. To screen the drugs which can reverse VCR resistance for VCR resistant cell strain, an analytical method has been established for the determination of VCR concentration in tumor cells using high performance liquid chromatography (HPLC). The stainless steel column was 25 cm x 4.6 mm i.d. packed with totally porous, spherical silica particles (5 microns). A solution of methanol and 0.02 mol/L dipotassium hydrogen phosphate (80:20, V/V) adjusted to pH 6.6 with H3PO4 was employed as the mobile phase. The flow rate was 1 mL/min. Chromatography was performed with ultraviolet detector at 267 nm. The method is simple, rapid and selective. Linear calibration curve for VCR was measured within the range of 10 to 200 mg/L with correlation coefficient of 0.999 8. The lowest detection limit was 4 mg/L tumor cells extract. The HPLC method described is suitable for clinical monitoring and pharmacokinetic study of VGR.