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Voltage-driven DNA translocations through a nanopore.

A Meller¹, L Nivon, D Branton

¹The Rowland Institute for Science, Cambridge, MA 02142, USA.

Physical Review Letters

|May 1, 2001

Summary

We measured DNA polymer translocation through a nanopore, finding shorter polymers move faster. This velocity depends non-linearly on the applied electric field, impacting diffusion and energy penalty estimations.

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Area of Science:

Nanopore sensing
Biophysics
Polymer physics

Background:

Single-stranded DNA (ssDNA) translocation through nanopores is a key process in DNA sequencing and analysis.
Understanding polymer dynamics within confined geometries like nanopores is crucial for developing advanced biosensing technologies.

Purpose of the Study:

To investigate the translocation dynamics of single-stranded DNA polymers through a single alpha-hemolysin pore.
To determine the velocity of polymers within the pore and its dependence on polymer length and applied electric field.
To estimate the effective diffusion coefficient and energy penalty associated with polymer extension into the pore.

Main Methods:

Measuring ionic current blockade and translocation time distributions for ssDNA polymers.
Utilizing voltage-driven translocations through a single alpha-hemolysin pore.
Analyzing translocation data to extract polymer velocity, diffusion coefficients, and energy penalties.

Main Results:

Polymer velocity within the pore is dependent on polymer length and applied electric field.
Longer polymers (> pore length) translocate at a constant velocity.
Shorter polymers exhibit increased velocity with decreasing length, showing a non-linear relationship with the applied field.

Conclusions:

The translocation velocity of ssDNA polymers is length-dependent and non-linearly related to the applied electric field.
These findings provide insights into the forces governing polymer dynamics in nanopores.
The study estimates effective diffusion coefficients and energy penalties, crucial for nanopore-based applications.

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