Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Is it truly necessary to achieve complete stone-free status in cases of staghorn calculi?

Actas urologicas espanolas·2025
Same author

Low-Dose High-Resolution TOF-PET Using Ionization-activated Multi-State Low-Z Detector Media.

Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment·2021
Same author

Cartilage Repair Capacity within a Single Full-Thickness Chondral Defect in a Porcine Autologous Matrix-Induced Chondrogenesis Model Is Affected by the Location within the Defect.

Cartilage·2021
Same author

Refining cage change routines: comparison of cardiovascular responses to three different ways of cage change in rats.

Laboratory animals·2011
Same author

Impact of aspen furniture and restricted feeding on activity, blood pressure, heart rate and faecal corticosterone and immunoglobulin A excretion in rats (Rattus norvegicus) housed in individually ventilated cages.

Laboratory animals·2009
Same author

Voltage-driven DNA translocations through a nanopore.

Physical review letters·2001

Related Experiment Video

Updated: Mar 9, 2026

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

14.2K

Single-Molecule Characterization of DNA-Protein Interactions Using Nanopore Biosensors.

A H Squires1, T Gilboa2, C Torfstein2

  • 1Stanford University, Stanford, CA, United States.

Methods in Enzymology
|January 8, 2017
PubMed
Summary
This summary is machine-generated.

Nanopore technology offers a sensitive, label-free method for studying DNA-protein interactions. This technique maps binding sites and measures interaction strength, advancing genetic regulation research.

Keywords:
DNA–protein interactionsForce spectroscopyLow-stress silicon nitrideNanoporeProtein–nucleic acid complexesSingle moleculeSingle-stranded DNA

More Related Videos

Monitoring Protein Adsorption with Solid-state Nanopores
08:51

Monitoring Protein Adsorption with Solid-state Nanopores

Published on: December 2, 2011

14.1K
Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

14.9K

Related Experiment Videos

Last Updated: Mar 9, 2026

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

14.2K
Monitoring Protein Adsorption with Solid-state Nanopores
08:51

Monitoring Protein Adsorption with Solid-state Nanopores

Published on: December 2, 2011

14.1K
Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

14.9K

Area of Science:

  • Biophysics
  • Molecular Biology
  • Genetics

Background:

  • Understanding genetic regulation requires characterizing nucleic acid-protein interactions.
  • Current methods face challenges in detecting and analyzing these crucial molecular complexes.
  • Nanopore sensing presents a sensitive, label-free approach for biomolecule analysis.

Purpose of the Study:

  • To advance nanopore sensing for detecting and characterizing DNA-protein interactions.
  • To explore nanopores for mapping protein binding sites on nucleic acids.
  • To utilize nanopore force spectroscopy for quantifying DNA-protein binding strengths.

Main Methods:

  • Utilizing nanopores as nanoscale apertures for single biomolecule detection.
  • Employing electrophoretic forces to draw charged molecules through the nanopore.
  • Developing dual sensing modes: binding site mapping and force spectroscopy.

Main Results:

  • Demonstrated nanopore capability for label-free detection of protein-nucleic acid complexes.
  • Successfully mapped binding site locations along DNA molecules.
  • Quantified DNA-protein interaction strengths using electrophoretic force and pore geometry.

Conclusions:

  • Nanopore sensing is a powerful tool for studying DNA-protein interactions at the single-molecule level.
  • The technique offers unique advantages for both mapping binding sites and measuring interaction forces.
  • Further development of nanopore technology will enhance our understanding of genetic regulation.