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Using Coculture to Detect Chemically Mediated Interspecies Interactions
Published on: October 31, 2013
Green marker for colonies of Bacillus subtilis
M Itaya1, S M Shaheduzzaman, K Matsui
1Mitsubishi Kasei Institute of Life Sciences, Machida-shi, Tokyo, Japan. ita@libra.ls.m-kagaku.co.jp
Abstract:
A Bacillus subtilis plasmid encoding a green fluorescence protein gene (gfp) was constructed. The fluorescence of B. subtilis colonies having this plasmid on agar plates was so high that they could be readily discerned visually under UV light. The fluorescence could be effectively expressed in three ways (i) through use of a strong bsr promoter (blasticidin S resistance gene), (ii) by efficient translation with the bsr translation system, and (iii) through increase in the copy number per cell. The high stability of the GFP plasmid was demonstrated by using more complicated growth conditions without any antibiotic for selection.
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