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Published on: May 4, 2012
Re-evaluation of the Anti-glycation Activity of Herbal Extracts Using Multiple Anti-glycation Assays
Mami Ishikawa1, Ryoji Taniguchi2, Taizo Taniguchi1,3
1Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, 7-1-20 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Hyogo, Japan.
Bioscience, Biotechnology, and Biochemistry
|July 27, 2026
Summary
Herbal extracts were tested for anti-glycation effects using multiple assays. Coptis, Cinnamomum, Connarus, and Pueraria showed distinct profiles in inhibiting advanced glycation end product (AGE) formation.
Area of Science:
- Biochemistry
- Pharmacology
- Natural Products Chemistry
Background:
- The glycation process, leading to advanced glycation end products (AGEs), contributes to diabetes-associated tissue damage.
- Herbal extracts are explored for antidiabetic and anti-glycation properties, but evaluation methods vary.
Purpose of the Study:
- To re-evaluate eight herbal extracts for their anti-glycation effects using both co-addition and post-addition assays.
- To distinguish between the inhibition of AGE formation and the modification of preformed AGEs.
- To reveal distinct anti-glycation profiles of herbal extracts.
Main Methods:
- Utilized glyceraldehyde-induced collagen and lysozyme glycation models.
- Employed both co-addition and post-addition assay strategies.
- Quantified AGE-derived fluorescence and analyzed protein dimerization.
Main Results:
- Coptis, Cinnamomum, Connarus, and Pueraria reduced AGE-derived fluorescence in the collagen model under both co- and post-addition conditions.
- In the lysozyme model, Cinnamomum, Connarus, and Pueraria reduced dimer bands, while Coptis had a limited effect.
- Multiple assays differentiated between inhibition of AGE formation and effects on preformed AGEs, showing distinct herbal extract profiles.
Conclusions:
- Multiple anti-glycation assays are crucial for accurately profiling herbal extracts.
- Distinct mechanisms of action exist among herbal extracts regarding glycation inhibition.
- This study provides a more comprehensive understanding of the anti-glycation potential of selected herbal extracts.
Keywords:
Advanced glycation end productsAnti-glycation activityHerbal extractsLysozyme glycationPost-addition modelSDS–PAGE
