Related Experiment Videos
Characterization of the ToxB gene from Pyrenophora tritici-repentis
J P Martinez1, S A Ottum, S Ali
1Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331, USA.
Molecular Plant-Microbe Interactions : MPMI
|May 3, 2001
Abstract:
The ToxB gene was cloned and characterized from a race 5 isolate of Pyrenophora tritici-repentis from North Dakota. ToxB contains a 261-bp open reading frame that encodes a 23 amino acid putative signal peptide and a 64 amino acid host-selective toxin, Ptr ToxB. Analysis of Ptr ToxB from heterologous expression in Pichia pastoris confirms that ToxB encodes a host-selective toxin.