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Developmental expression patterns of the signaling adapters FRS-2 and FRS-3 during early embryogenesis

K McDougall1, C Kubu, J M Verdi

  • 1Neurodegeneration Group, The John P. Robarts Research Institute, 100 Perth Drive, London, Ontario, N6A 5K8, Canada.

Mechanisms of Development
|May 4, 2001
PubMed

Insights

Fibroblast growth factor receptor substrates 2 and 3 (FRS2 and FRS3) are crucial for embryonic development. This study maps their expression patterns in developing and adult mice, revealing distinct roles in embryogenesis.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Cell Signaling

Background:

  • Fibroblast growth factor (FGF) and neurotrophin signaling are vital during embryogenesis.
  • Fibroblast growth factor receptor substrates (FRS2 and FRS3) mediate downstream signaling from FGF and Trk receptors.
  • Expression patterns of FRS2 and FRS3 during murine development are currently unknown.

Purpose of the Study:

  • To characterize the spatiotemporal expression of FRS2 and FRS3 during mouse embryonic development (E7 to parturition).
  • To determine the expression profiles of FRS2 and FRS3 in adult murine tissues.

Main Methods:

  • Whole-mount in situ hybridization was used to detect FRS2 and FRS3 expression in mouse embryos.
  • Immunohistochemistry was employed to analyze protein localization in fetal and adult tissues.

Main Results:

  • Both FRS2 and FRS3 were detected in E8.5 mouse embryos.
  • FRS2 expression was observed early (E7) in the syncytiotrophoblast, neural tube, and various fetal/adult tissues.
  • FRS3 expression was more restricted, found in the fetal neural tube, heart, stomach, liver, and predominantly in the ventricular layer of the developing neural tube and brain.

Conclusions:

  • FRS2 and FRS3 exhibit distinct expression patterns throughout murine development.
  • These differential expression profiles suggest specialized roles for FRS2 and FRS3 in embryogenesis and tissue-specific functions.

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