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Virus-cell interactions as seen by scanning electron microscopy.

H Gamliel, A Polliack

    Israel Journal of Medical Sciences
    |August 1, 1979
    PubMed
    Summary
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    Scanning electron microscopy (SEM) reveals virus-induced cell surface changes, including cell rounding and microprojection development. Future studies using sensitive SEM methods can better characterize these viral effects on cell morphology.

    Area of Science:

    • Cell Biology
    • Virology
    • Microscopy

    Background:

    • Scanning electron microscopy (SEM) is a valuable tool for examining cell surface topography.
    • Viral infections are known to induce significant alterations in host cell morphology.
    • Previous SEM studies have documented various surface changes in virus-infected cells.

    Purpose of the Study:

    • To review the application of SEM in identifying virus-induced cell surface alterations.
    • To summarize common morphological changes observed in virus-infected cells using SEM.
    • To propose future directions for SEM-based research in virology.

    Main Methods:

    • Utilizing scanning electron microscopy (SEM) to visualize and analyze the surface of infected cells.
    • Comparing surface morphology of virus-infected cells with control cells.

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  • Reviewing existing literature on SEM studies of viral infections.
  • Main Results:

    • SEM consistently reveals dramatic shape changes in virus-infected cells.
    • Common alterations include cell rounding and the formation of surface microprojections like ruffles and microvilli.
    • These changes are observed across various cell types infected with different RNA and DNA viruses.

    Conclusions:

    • SEM is effective in detecting virus-induced cellular surface modifications.
    • Further development of sensitive SEM methodologies is recommended for enhanced characterization of viral cytopathic effects.
    • Future research should focus on refining SEM techniques to better understand virus-host interactions at the cellular level.