Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Directed gap closure in large-scale sequencing projects.

M Frohme1, A A Camargo, C Czink

  • 1Functional Genome Analysis, Deutsches Krebsforschungszentrum, Heidelberg, Germany. m.frohme@dkfz-heidelberg.de

Genome Research
|May 5, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Signal and Time Resolved Information Experiment (SATIRE): An NMR Supersequence for Monitoring Complex Environmental and Biological Processes without <sup>13</sup>C Enrichment.

Analytical chemistry·2025
Same author

Demonstration of ultra-high dose rate electron irradiation at FLASH<i>lab</i>@PITZ.

Physics in medicine and biology·2025
Same author

Pooled prevalence of lymphopenia in all-cause hospitalisations and association with infection: a systematic review and meta-analysis.

BMC infectious diseases·2023
Same author

Environmental metabolomics uncovers oxidative stress, amino acid dysregulation, and energy impairment in Daphnia magna with exposure to industrial effluents.

Environmental research·2023
Same author

Diagnosing ventilator-associated pneumonia (VAP) in UK NHS ICUs: the perceived value and role of a novel optical technology.

Diagnostic and prognostic research·2022
Same author

Donor monocyte-derived macrophages promote human acute graft-versus-host disease.

The Journal of clinical investigation·2020
Same journal

A unified analysis of cell type- and trajectory-associated pathways in single-cell data using Phoenix.

Genome research·2026
Same journal

Resf1 is required for proper placental development and configuration of trophoblast cell-specific heterochromatin.

Genome research·2026
Same journal

Telomere-driven replicative crisis is driven by large-scale changes in genomic architecture.

Genome research·2026
Same journal

Spatially informed reference-free cell-type deconvolution for spatial transcriptomics with SpatialCD.

Genome research·2026
Same journal

Spatially resolved profiling of steroid nuclear receptors reveals a role for the disordered N-terminal domains in genome targeting and AP-1 interaction.

Genome research·2026
Same journal

Flexible and scalable inference of spatially varying correlation in spatial transcriptomics with spCorr.

Genome research·2026
See all related articles

Researchers developed a method to close sequencing gaps using subtractive hybridization. This technique uses clone libraries to target uncloned genomic regions, aiding in complete genome sequencing.

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Sequencing projects often face challenges with gaps in clone libraries due to uncloned genomic regions.
  • These gaps hinder the completion of whole genome sequencing efforts.

Purpose of the Study:

  • To present a directed approach for generating sequence information from gaps in clone libraries.
  • To provide a method for achieving complete genome closure in sequencing projects.

Main Methods:

  • Utilizing the Xylella fastidiosa genome as a model system.
  • Employing subtractive hybridization, similar to representational difference analysis (RDA).
  • Using the complete clone library as a competitor against genomic DNA of interest.

Main Results:

Related Experiment Videos

  • Successfully generated directed sequence information from previously inaccessible genomic areas.
  • Demonstrated the efficacy of the subtractive hybridization approach for gap filling.

Conclusions:

  • The proposed method effectively addresses the challenge of sequencing gaps.
  • This approach facilitates selective screening of clone resources and direct gap closure for complete genome sequencing.