[Evaluation of the use of isoelectrofocusing method for measuring glycated hemoglobin]

Measurement of HbA1c is important for the diagnosis of early forms of diabetes mellitus, hemolytic and sickle-cell anemia, as it can be used for optimal and permanent monitoring of hyperglycemia and serve as an indicator of hemolysis process. Isoelectrofocussing method (IEF) was modified for measuring HbA1c in polyacrylamide gel (pH 3.5-9.5) on an LKB multiphore (Sweden). HbA1c was measured in 350 patients with diabetes mellitus, sickle-cell and hemolytic anemia, and in 120 donors. In diabetics the mean content of HbA1c was 9.2 +/- 1.2%, in patients with hemolytic anemia 3.8 +/- 0.2%, and in patients with sickle-cell anemia 3.9 +/- 0.28%, vs. the normal value of 5.2 +/- 0.13%. Hence, our experiments demonstrated the possibility of wide use of IEF in our modification for identification of the above diseases in clinical laboratory.