Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evolutionary relationships among Rel domains indicate functional diversification by recombination.

I A Graef1, J M Gastier, U Francke

  • 1Department of Genetics, Howard Hughes Medical Institute, Stanford University Medical School, Stanford, CA 94305-5323, USA.

Proceedings of the National Academy of Sciences of the United States of America
|May 10, 2001
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synaptic Activity Causes Minute-scale Changes in BAF Complex Composition and Function.

bioRxiv : the preprint server for biology·2023
Same author

'Nature-inspired' drug-protein complexes as inhibitors of Abeta aggregation.

Biochemical Society transactions·2005
Same author

NFAT signaling in vertebrate development.

Current opinion in genetics & development·2001
Same author

Signals transduced by Ca(2+)/calcineurin and NFATc3/c4 pattern the developing vasculature.

Cell·2001
Same author

Monitoring the duration of antigen-receptor occupancy by calcineurin/glycogen-synthase-kinase-3 control of NF-AT nuclear shuttling.

Current opinion in immunology·2001
Same author

Cell signaling can direct either binary or graded transcriptional responses.

The EMBO journal·2001

Genomic analysis reveals how rel domains evolved into distinct protein families, including NF-kappaB, NFATc, and TonEBP. Vertebrate-specific NFATc proteins likely evolved for unique functions like immune response and nervous system development.

Area of Science:

  • Evolutionary genomics
  • Molecular biology
  • Comparative genomics

Background:

  • Genomic sequencing enables tracking gene family evolution via genomic structure.
  • Understanding exon-domain associations in invertebrates aids predicting mammalian multidomain protein functions.

Purpose of the Study:

  • Determine the genomic structure of 14 invertebrate and vertebrate genes containing rel domains.
  • Investigate the evolutionary recombination of rel domains with distinct genomic sequences.

Main Methods:

  • Comparative genomic analysis of rel domain-containing genes across species.
  • Identification of intronic boundaries defining the rel domain sequence.
  • Analysis of gene recombination events and protein family evolution.

Related Experiment Videos

Main Results:

  • The rel domain recombined with distinct sequences to form rel/Dorsal/NFkappaB, NFATc, and TonEBP proteins.
  • A single exon in NFATc genes encodes the entire calcium-sensing region.
  • Rel/Dorsal and TonEBP proteins are in Drosophila but not C. elegans; NFATc proteins are vertebrate-specific.

Conclusions:

  • The NFATc family's vertebrate-specific presence suggests roles in vertebrate-specific functions.
  • These functions may include immune response, cardiovascular development, and nervous system development.